ABSTRACT
<p><b>OBJECTIVE</b>To optimize formulation of tanshinone II(A)-loaded PLGA nanoparticles and compare the difference of two methods in preparation and quality of nanoparticles.</p><p><b>METHOD</b>The two methods were nanoprecipitation method and emulsion-evaporation method. Single factor experiments and central composite design and response surface method were used to optimize the formulation of nanoparticles. The nanoparticles were characterized at size, morphology, entrapment efficiency, drug loading, drug recovery rate, crystallinity and drug release in vitro.</p><p><b>RESULT</b>The mean diameters were 225 nm and 183 nm, the entrapment efficiency were 95.49% and 87.99%, the drug loading were 2.03% and 0.16%, and the drug recovery rates were 38.42% and 17.59% respectively for nanoprecipitation method and emulsion-evaporation method.</p><p><b>CONCLUSION</b>Nanoprecipitation method was better than emulsion-evaporation method for preparation of tanshinone II(A)-loaded PLGA nanoparticles.</p>
Subject(s)
Chemical Precipitation , Crystallization , Abietanes , Emulsions , Lactic Acid , Chemistry , Nanoparticles , Chemistry , Particle Size , Phenanthrenes , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Polyglycolic Acid , Chemistry , Polymers , Chemistry , Quality Control , Salvia miltiorrhiza , Chemistry , Technology, Pharmaceutical , Methods , VolatilizationABSTRACT
<p><b>OBJECTIVE</b>Study the characteristics of absorption and separation of traditional Chinese medicine compound prescription using macroporous resin.</p><p><b>METHOD</b>Study the techniquecs and characteristics of absorption and separation of a sample by macroporous resin, which is composed of coptis root, rhubarb and common anemarrhena rhizome, containing alkaloid, anthraquinone and saponin.</p><p><b>RESULT</b>It is proved by qualitative and quantitative researches studies that after absorbed and separated by optimized technics process, most prime effective components or section fractions in traditional Chinese medicine compound prescription can be reserved maintained.</p><p><b>CONCLUSION</b>If the techniquecs of separation is properly designed, the same kind of macropore resin can absorbd and separate various effective components or section in traditional Chinese medicine compound prescription which have with different chemical structures efficiently.</p>
Subject(s)
Alkaloids , Anemarrhena , Chemistry , Anthraquinones , Coptis , Chemistry , Plants, Medicinal , Chemistry , Resins, Synthetic , Rheum , Chemistry , Saponins , Technology, Pharmaceutical , MethodsABSTRACT
<p><b>OBJECTIVE</b>To study the absorption and separation of different glycoside on different macropore resins.</p><p><b>METHOD</b>Take baikal skullcap root, cape jasmine fruit and white peony root as samples and study the different characterstics of absorption and separation of these samples on macropore resins such as D101 and so on.</p><p><b>RESULT</b>The static absorption effect of the the three aglycones on six different macropore resins is baicalin > lactiflorin > gardoside. Their elution are 75% CH3OH, 25% CH3OH, and 45% CH3OH. Their elution rates are 60%, 93%, and 93%.</p><p><b>CONCLUSION</b>Similar molecules may not have similar absorption abilities on same macropore resins, but the effect of absorption has something to do with the structures of the molecules, the more double-bonds the molecules have, the greater the absorption force the resins have.</p>
Subject(s)
Absorption , Flavonoids , Chemistry , Fruit , Chemistry , Glycosides , Chemistry , Jasminum , Chemistry , Molecular Structure , Paeonia , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Resins, Plant , Scutellaria baicalensis , ChemistryABSTRACT
<p><b>AIM</b>To study the preparation of bovine serum albumin nanoparticles surface-modified with glycyrrhizin(BSA-NP-GL) targeting to hepatocytes.</p><p><b>METHODS</b>The bovine serum albumin nanoparticles (BSA-NP) were prepared by desolvation process. Glycyrrhizin (GL) was oxidized by sodium periodate to be conjugated to surface reactive amino groups (SRAG) of the BSA-NP. The SRAG were quantified by spectrophotometric method using 2, 4, 6-trinitrobenzenesulfonic acid(TNBS). Glycyrrhetinic acid(GA) hydrolyzed from GL, which was on the surface of BSA-NP-GL was assayed by HPLC after isolation by sephadex G-50. Both methods were used to verify the conjugation achieved. HPLC was used to determine surface density of GL on BSA-NP-GL.</p><p><b>RESULTS</b>The amount of SRAG of the BSA-NP-GL decreased by 19.6% compared with normal BSA-NP. The amount of GL molecule was 9.2% of the total determined SRAG of BSA-NP. The mean diameter of the BSA-NP-GL was 73 nm with round shape. The stability of BSA-NP-GL was constant when it was stored at 25 degrees C and 37 degrees C during 10 days.</p><p><b>CONCLUSION</b>BSA-NP-GL was successfully prepared, which is considered to establish an experimental foundation for further research on its ability for targeting to hepatocytes.</p>